The effect of temperature on the energy budget of the Manila clam, <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>

In this study, the energy budget of the Manila clam, Ruditapes philippinarum, was evaluated after one-week acclimation periods at 5, 10, 15, 20, and 25°C. Small clams (151 ± 12 mg DW) and large clams (353 ± 16 mg DW) were fed with the microalgae, Isochrysis galbana. Filtration rate, ingestion rate, assimilation efficiency, oxygen-consumption rate, and ammonia excretion rate were measured. Both filtration rate and ingestion rate of small and large clams were found to be related to temperature. The highest Q ₁₀ values were measured in the range 15–20°C for both small and large clams. Assimilation efficiency of both small and large clams was not significantly influenced by temperature, although the maximum mean values were detected at 20°C. Oxygen consumption rate and ammonia excretion rate of small and large clams were found to be related directly to temperature over the entire range, with a maximum being detected at 25°C. The highest Q ₁₀ value was estimated in the range 10–15°C with regard to oxygen consumption rate, and in the range of 15–20°C with regard to ammonia excretion rate. Scope for growth (SFG) was positive at all temperatures, achieving a maximum value at 20°C in both small and large clams, primarily as a consequence of the enhanced ingestion rate which offset the concomitant elevation in the metabolic rate. In this study we have estimated the thermal optimum for this species at 20°C.     

日光温室果树台田式限根栽培温度变化规律的研究

通过对日光温室台田式限根槽栽培方式的地温与气温变化规律研究.结果表明:温室内气温和地温最低值出现时间一致;气温变化快,日变幅大;地温受气温影响,变化明显滞后;随着土层加深,地温日变幅变小;升温初,台田较平作地温上升快.覆盖白色和黑色地膜都能明显提高地温.土层越浅并且距离限根槽边缘越近,地温受气温影响越大;随土层加深各部位地温差减小.台田式限根栽培有利于迅速提高地温,协调地温和气温关系.     

Pt100铂热电阻在雨花茶精揉机温度控制中的应用

从铂热电阻非平衡电桥测温原理出发,分析了雨花茶精揉机揉锅温度测量与控制系统在温度信号采集过程中引入的非线性误差,提出了以Pt100铂热电阻和AT89S52单片机为核心的软件非线性补偿方案,给出了系统硬件结构图和软件设计流程图。实验结果表明该方案简便有效,能够提高温度控制系统性能。     

The combined effects of temperature and GnRHa treatment on the final stages of sexual maturation in Atlantic salmon (Salmo salar L.) females

Atlantic salmon (Salmo salar L.) females (2 SW), maturing for the first time, were reared under one of three temperature regimes (high: 14.3 ± 0.5°C; natural: 10.6 ± 1.0°C; and cold: 6.9 ± 1.0°C) in combination with one of two experimental treatments; an injection of GnRH analogue (GnRHa) contained in biodegradable microspheres, or a sham injection (microspheres only). The six experimental groups were then reared under simulated natural photoperiod for 4 weeks. Blood samples were drawn for analysis of plasma steroid levels and the fish were inspected for ovulation weekly. Batches of stripped eggs were incubated in triplicate incubators in raceways until the eyed stage. Treatment with GnRHa resulted in a substantial advancement and synchronization of ovulation at all temperatures, while exposure to cold water also appeared to advance ovulation slightly. While 75% (warm and cold) to 90% (natural) of GnRHa fish ovulated during the 4-week trial, only 30% of sham-treated females exposed to cold water, and none of the sham-treated fish held at higher temperatures, ovulated during this period. Survival rates of embryos to the eyed-stage were significantly higher for broodstock exposed to cold water. Plasma levels of testosterone (T), 17β-oestradiol (E₂), and 17α,20β-dihydroxy-4-pregnen-3-one (17,20βP) were all significantly affected by treatment with GnRHa and, to a lesser extent, temperature. The efficiency of GnRHa in counteracting the negative effects of high temperature on ovulation and the associated changes in circulating sex steroids suggest that temperature inhibition operates at least in part at the brain or pituitary.     

DNA recovery from agarose gels with a simple centrifuge-driven sephadex filtration

Conventional methods of DNA recovery from agarose gel generally require expensive equipment, extended elution times, or considerable handling of the sample after elution. We developed a simple protocol for a quick and effective recovery of DNA from agarose gels with good yield and quality. Using a Sephadex resin filled spin column, DNA fragments of 500 bp to 6 kb in an agarose gel slice were easily recovered by a 2 min centrifugation. The recovery efficiencies were over 40%-50% and the eluted DNA can be used directly for downstream application, such as polymerase chain reactions (PCR) and restriction enzyme digestion. This method could also be used to recover large DNA fragment (48 kb) without degradation. The use of Sephadex helps to remove small molecular impurities from agarose and it also reduces the chance of clogging the column filter caused by direct contact with agarose.     

小叶白掌变异苗的恢复

小叶白掌变异苗的恢复应在25℃一28℃条件下。对4类变异苗，以MS IAA0．1mg／L培养基的效果为好；对5类变异苗则以MS IBA0.3mg/L培养基较好。     

Effects of detomidine or romifidine during maintenance and recovery from isoflurane anaesthesia in horses

ObjectiveTo evaluate the effects of detomidine or romifidine on cardiovascular function, isoflurane requirements and recovery quality in horses undergoing isoflurane anaesthesia.Study designProspective, randomized, blinded, clinical study.AnimalsA total of 63 healthy horses undergoing elective surgery during general anaesthesia.MethodsHorses were randomly allocated to three groups of 21 animals each. In group R, horses were given romifidine intravenously (IV) for premedication (80 μg kg^–1), maintenance (40 μg kg^–1 hour^–1) and before recovery (20 μg kg^–1). In group D2.5, horses were given detomidine IV for premedication (15 μg kg^–1), maintenance (5 μg kg^–1 hour^–1) and before recovery (2.5 μg kg^–1). In group D5, horses were given the same doses of detomidine IV for premedication and maintenance but 5 μg kg^–1 prior to recovery. Premedication was combined with morphine IV (0.1 mg kg^–1) in all groups. Cardiovascular and blood gas variables, expired fraction of isoflurane (Fe′Iso), dobutamine or ketamine requirements, recovery times, recovery events scores (from sternal to standing position) and visual analogue scale (VAS) were compared between groups using either anova followed by Tukey, Kruskal-Wallis followed by Bonferroni or chi-square tests, as appropriate (p < 0.05).ResultsNo significant differences were observed between groups for Fe′Iso, dobutamine or ketamine requirements and recovery times. Cardiovascular and blood gas measurements remained within physiological ranges for all groups. Group D5 horses had significantly worse scores for balance and coordination (p = 0.002), overall impression (p = 0.021) and final score (p = 0.008) than group R horses and significantly worse mean scores for VAS than the other groups (p = 0.002).Conclusions and clinical relevanceDetomidine or romifidine constant rate infusion provided similar conditions for maintenance of anaesthesia. Higher doses of detomidine at the end of anaesthesia might decrease the recovery quality.     

云南岩溶地区石漠化生态恢复的思路

云南岩溶面积达11.09万km2,占全省国土面积的28.14%,是西南地区岩溶集中分布区,岩溶石漠化面积已达2.15万km2.分析云南岩溶的分布,以及岩溶地区生态脆弱给当地人民带来生产、生活的生态危机,并结合云南石漠化治理的成功经验,提出岩溶地区生态恢复的思路.     

次生阔叶林改建为食用菌原料林后的群落恢复和林分生长

选择12年生林相较好的天然次生阔叶林,通过择伐更新和皆伐人促更新2种经营方式定向培育食用菌原料林。研究发现,择伐经营的经济效益较高,且有利于群落的快速恢复,经过7a的封育,林分蓄积量已达到择伐前的水平。皆伐人促更新获得的食用菌材虽较择伐利用高出40%左右,但因经营强度过大,保留的幼树受损严重而不利于伐后林木的生长与恢复,封育期内蓄积增长量分别为择伐更新和对照未经营区的64 7%和73 41%。动态监测结果显示,采用择伐和皆伐人促更新措施后群落恢复较快。疏伐后因透光度的急剧增加,阳性和中性树种大量侵入,地面芽植物和1年生植物的比例增高。到第7a时,高位芽植物的比例已接近未经营区的状态,乔、灌、草各层的物种多样性指数高于未经营的天然阔叶林,物种均匀度则与其相近。研究还发现,2种更新经营方式对土壤养分的损失较小,经过7a的恢复,林地土壤肥力已基本达到原有天然林水平。     

植被恢复与重建过程中小流域降雨及水沙变化特征研究

以女儿寨小流域为研究对象，着重研究了植被恢复与重建过程中小流域系统的降雨和水沙变化特征，并对植被恢复过程中的总体减沙水平做了分析。结果表明：①受大气环流的影响，小流域年际降雨量差异较大；小流域降雨年内时间分布极为不均，集中性很大，主要集中在汛期（4-8月），但年内降雨量的月间分布趋势基本一致。②小流域迳流年际变化很大，迳流年内分配不均。迳流集中是该小流域迳流变化的一个显著特征：小流域的植被恢复与重建对洪峰的出现和洪峰流量具有明显的延缓和削减作用。③径流（Qi）主要来源于降水（Pi），二者呈明显的一元线型关系：Pi=115．7678＋2．7386Qi。④小流域的年输沙模数与年迳流量的变化规律基本一致，小流域年内产沙也高度集中，且与降水、迳流关系密切，小流域的土壤侵蚀主要由汛期的强降水引起。⑤随着小流域大面积、高标准的生态环境建设，1990年至2004年间小流域的输沙模数呈锐减之势，植被恢复与重建起到了显著的减沙效应。